ClonExpress II One Step Cloning Kit 618.00 Phanta Max Super-Fidelity DNA Polymerase 618.00 615.00 2 Phanta Max Master Mix 615.00 700.00 Ultra GelRed (10000 ) 700.00 60.00 DL2000 Plus DNA Marker 60.00 60.00 DL5000 DNA Marker 60.00 75.00
2016-8-5 · ClonExpress II One Step Cloning Kit (Vazyme Biotech Co. Ltd). Typically 20 μL of a mixture containing 4 μL of 5 CE II Buffer 2 μL of Exnase II 50 ng linear plasmid 100 ng PCR fragment and ddH2O was incubated at 37oC for 30 min and the resulting solution was used to transform chemically competent E. coli DH5α cells. Colony PCR and
ClonExpress II One Step Cloning Kit. 73.00 €122.00 €. excl. VAT plus shipping. Size. Choose an option 25 RXNS 50 RXNS. Clear. ClonExpress II One Step Cloning Kit quantity. Add to cart. Categories Cloning Mutagenesis Fast Cloning Molecular Biology SKU C112.
2021-7-20 · ClonExpress ® DNA ClonExpress ® II Exnase ® II
2021-2-8 · The purified PCR products were fused to the linearized pRGEB32- GhU6.9 vector using ClonExpress II One Step Cloning Kit. Each assembly group should contain at least 200 monocolonies of Bacterial-host-competent cells grown in a selection medium. All resistant cells were then harvested in 100 mL lysogeny broth (LB) medium and cultured over night
2021-7-9 · ClonExpress II One Step Cloning Kit 25 rxn. Home » Shop » ClonExpress II One Step Cloning Kit 25 rxn. ClonExpress® technology is a simple fast and efficient DNA seamless cloning technology which can clone the insert into any position of any vector. Linearize the vector and place the insert 5 in the forward/reverse PCR primer Introduce
ClonExpress® II One Step Cloning Kit. . 95 . 50 bp-10 kbp DNA. PCR. .
As a new generation recombinant cloning kit ClonExpress® II has a unique non-ligase dependent system which greatly reduces the background of vector
ClonExpress II One Step Cloning Kit C112-02 -Equl_AdvancedBioMatrix_DivBio_Drummond_Genie_Glascol_Megazyme_Phadebas_Worthington Megazyme AUVON ElisaSystems Phadebas Phadebas EBPI TDzyme
ClonExpress II One Step Cloning Kit and Phanta Max (p515) DNA polymerases were purchased from Vazyme Biotech Co. Ltd. (Nanjing China). Oligonucleotides were synthesized by GENEWIZ Bio Inc. (Suzhou China). Strains plasmids and media. The strains and plasmids involved in this study are listed in table S3. The MP6 and CRISPR systems for gene
2017-11-14 ·
ClonExpress II One Step Cloning Kit C112-01/02 600/1000 1971
The fragment was ligated into the HindIII-digested mini-CTX-lacZ plasmid following the instruction of the ClonExpress II One Step Cloning Kit (Vazyme China) generating mini-CTX-Ptat-lacZ. The I-F cas operon including its native promoter (8.693-kb) was obtained by PCR employing PA154197 genomic DNA as the template.
ClonExpress II One Step Cloning (Vazyme #C112) is a simple fast and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly the vector is linearized at the cloning
As a new generation recombinant cloning kit ClonExpress® II has a unique non-ligase dependent system which greatly reduces the background of vector self-ligation and the positive rate can reach more than 95 . The highly optimized reaction buffer and the enhanced recombinase Exnase II significantly improve the recombination efficiency of
2020-2-3 · PCR products were inserted into lined pART27 GFP using ClonExpress II One Step Cloning Kit (Vazyme China). These positive clones were transformed into Agrobacterium tumefaciens strain GV3101. The pART27 GFP transformed Agrobacterium
2021-3-15 · C112 ClonExpress II One Step Cloning kit.pdf Created Date 6/5/2019 1 12 08 PM
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2020-8-7 · ClonExpress ® II Exnase ® II PCR5- PCR53 15bp 20bp
ClonExpress II One Step Cloning Kit Vazyme 25 rxn/50 rxn C112-01/02 600/1000
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2021-6-16 · ClonExpress®II Exnase®II / PCR 5 PCR 5 3 (15 bp-20 bp)
ClonExpress® II One Step Cloning Kit. . 95 . 50 bp-10 kbp DNA. PCR. .
2016-8-5 · ClonExpress II One Step Cloning Kit (Vazyme Biotech Co. Ltd). Typically 20 μL of a mixture containing 4 μL of 5 CE II Buffer 2 μL of Exnase II 50 ng linear plasmid 100 ng PCR fragment and ddH2O was incubated at 37oC for 30 min and the resulting solution was used to transform chemically competent E. coli DH5α cells. Colony PCR and
2017-4-13 · The SPpr1-eGFP-T35S fragment was subsequently cloned into the SpeI/XbaI sites of Sol2095 with the ClonExpress II One-Step Cloning Kit yielding the plasmid pLOCGex (Fig. S2A). Another binary vector pLOCG without plant PR1 signal peptide was generated by amplification of the eGFP-T35S cassette with the primers GFP_pBin_F and T35S_pBin_R using
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