2013-9-13 · TOWARDS A MICROFLUIDIC SINGLE-CELL IMMUNE CHIP M. Junkin1 A. J. Kaestli1 and S. Tay1 1Department of Biosystems Science and Engineering ETH Zürich SWITZERLAND ABSTRACT We have designed a PDMS based microfluidic system
2015-8-25 · Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns. Nat Protoc. 2012 7 829–838. doi 10.1038/nprot.2012.021. Google Scholar 29. Hamacher-Brady A Brady NR Gottlieb RA. Enhancing macroautophagy protects against ischemia/reperfusion injury in cardiac myocytes. J Biol Chem.
2003-5-14 · microfluidic network enables the passive and gentle separation of a single cell from the bulk cell suspension and integrated valves and pumps enable the precise delivery of nanoliter volumes of reagents to that cell. Various applications are demonstrated including cell viability assays ionophore-mediated intracellular Ca2
2019-8-29 · The microfluidic system provides advanced technology for single-cell culture and observation. This chapter gives a brief account of single-cell capture by microfluidic methods long-term single-cell culture on both two-dimensional models and three-dimensional microfluidic systems as well as single-cell growth and differentiation in a
2013-11-15 · The paucity of single-cell RT-PCR studies has been attributed to the laborious and difficult task of purifying mRNA from individual cells and subsequently synthesizing and purifying total single-cell cDNA.17 The difficulty is due to the loss of material during the subsequent steps of cell isolation lysis mRNA isolation and cDNA synthesis.
2017-4-19 · Single-cell microfluidic approaches In recent years scientists have adapted micromanipu-lation schemes and microfluidic devices to address concerns of efficiency cost and labour in single-cell preparation and analysis. The fundamental elements of these devices are typically valves droplets or nanolitre-Measure scale wells (nanowells).
2021-5-20 · Single-cell growth inside the multichannel microfluidic device was observed for 18 h and images were obtained hourly. M. smegmatis cells were loaded into
2015-8-25 · Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns. Nat Protoc. 2012 7 829–838. doi 10.1038/nprot.2012.021. Google Scholar 29. Hamacher-Brady A Brady NR Gottlieb RA. Enhancing macroautophagy protects against ischemia/reperfusion injury in cardiac myocytes. J Biol Chem.
2019-5-8 · The microfluidic approach offers a rapid accurate and cost-effective tool for single-cell biology. We present an overview of the recent developments in microfluidic technology for chemical-content analysis of individual cells.
Microfluidic approaches for single-cell isolation Isolation is the first step for working with individual cells. Isolation is the process at which the target cells are separated and isolated to be manipulated or analyzed. Conventionally it was done using FACS machines that require expensive and bulky equipments as well as trained personnel.
2019-9-23 · Recently microfluidics has evolved to be a powerful technology for single-cell omics analysis due to its merits of throughput sensitivity and accuracy. Herein the recent advances of microfluidic single-cell omics analysis including different microfluidic platform designs lysis strategies and omics analysis techniques are reviewed.
2018-4-26 · A single-cell includes the minute quantity of these target molecules. Microfluidic devices have the ability to capture a single-cell and its lysate into a pico or femtoliter volumes droplet or micro-well thus preventing dilution and limiting diffusion. In this review we described the advancement and limitations in microfluidic techniques used
2019-10-30 · Microfluidic Platforms for Single-Cell Protein Analysis Yanli Liu1 and Anup K. Singh1 Abstract Elucidation of the heterogeneity of cells is a challenging task due to the lack of efficient analytical tools to make measurements with single-cell resolution. Microfluidics has emerged as a powerful platform for single-cell analysis with the ability to
2017-5-5 · Microfluidic single-cell analysis pipeline a Steps 1–15 Microfluidic chip fabrication and control system setup Setup Chip preparation surface treatment and cell loading b Steps 16–31 Chip loading Multiplexed stimulation and live imaging c Steps 32–43 Stimulation and imaging Cell retrieval and gene expression analysis
2017-4-19 · Single-cell microfluidic approaches In recent years scientists have adapted micromanipu-lation schemes and microfluidic devices to address concerns of efficiency cost and labour in single-cell preparation and analysis. The fundamental elements of these devices are typically valves droplets or nanolitre-Measure scale wells (nanowells).
2005-10-4 · Multiple cell types can thus be sequentially and selectively captured on desired electrodes. Single-cell capture efficiency is optimized by varying the duration of field application. Maximum single-cell capture is attained for the 10-min trial with 63 ± 9 (n = 30) of the electrode pad rows having a single cell.
2012-8-1 · Microfluidics provides cost-effective high-throughput single-cell analysis. Small volumes increase the concentration of template DNA RNA or secreted proteins. Microfluidic RT-qPCR arrays perform multiple gene measurements in individual cells. Automated microfluidic cell culture allows single cell tracking and dynamic stimulation. Applications in protein or signaling studies and personal and
2005-10-4 · Multiple cell types can thus be sequentially and selectively captured on desired electrodes. Single-cell capture efficiency is optimized by varying the duration of field application. Maximum single-cell capture is attained for the 10-min trial with 63 ± 9 (n = 30) of the electrode pad rows having a single cell.
2020-3-6 · For single-cell RT-PCR or qPCR workflows more stages can be integrated. Mary et al. described a single-cell qPCR microfluidic design for 100 samples that first encapsulates cells in droplets with lysis reagents followed by thermal cycling directly on-chip in droplets. Given the transparent property of Polydimethylsiloxane (PDMS) the
2020-12-22 · Single-cell analysis to investigate cellular heterogeneity and cell-to-cell interactions is a crucial compartment to answer key questions in important biological mechanisms. Droplet
2003-5-14 · microfluidic network enables the passive and gentle separation of a single cell from the bulk cell suspension and integrated valves and pumps enable the precise delivery of nanoliter volumes of reagents to that cell. Various applications are demonstrated including cell viability assays ionophore-mediated intracellular Ca2
2017-9-22 · manipulation necessary to isolate a single cell from a suspended population or tissue sample and effects of contamination which are amplified with such few RNA transcripts in a single cell. Here we present a microfluidic-based system to prepare cDNA from single cells for RNA sequencing with improved precision and sensitivity.
After a single MDA--231 breast cancer cell had adhered to the chamber of the microfluidic chip and been selected for based on size and morphological criteria the observation aperture for fluorescence measurement was adjusted and two detection boxes which equated to a size approximately 1 cm larger than the cell were marked on the TV
2020-3-6 · Microfluidic designs with their advantages of being high throughput low in reaction volume and compatible with bio-inert materials have been widely used to improve single-cell workflows in all major stages of single-cell applications from cell sorting to lysis to sample processing and readout.
2018-4-26 · A single-cell includes the minute quantity of these target molecules. Microfluidic devices have the ability to capture a single-cell and its lysate into a pico or femtoliter volumes droplet or micro-well thus preventing dilution and limiting diffusion. In this review we described the advancement and limitations in microfluidic techniques used
2013-11-15 · The paucity of single-cell RT-PCR studies has been attributed to the laborious and difficult task of purifying mRNA from individual cells and subsequently synthesizing and purifying total single-cell cDNA.17 The difficulty is due to the loss of material during the subsequent steps of cell isolation lysis mRNA isolation and cDNA synthesis.
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